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资讯资讯 / news 您的位置:球赛下注 > 资讯资讯 > 18080-044反转录酶III现货

18080-044反转录酶III现货

发布时间: 2016-02-22  点击次数: 1044次

18080-044反转录酶III现货 原装,质量保证,现货供应 2000元

SuperScript® III Reverse Transcriptase
货号:18080-044
规格:10000units
英文名称:SuperScript III Reverse Transcriptase
品牌:Invitrogen原装

18080-044反转录酶III现货 产品描述:

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.

• Thermostability – Half life of 220 minutes at 50°C
• Yield – Reduced RNase H activity for more full-length cDNA
• Specificity – Full activity at 50°C for increased specificity with Gene Specific Primers
• Applications – Array labeling, cDNA libraries, RT-PCR, primer extension, and 3´ and 5´ RACE

Source: Purified from E. coli expressing the pol gene of M-MLV (1, 2), mutagenized to increase thermal stability and half life and reduce RNase H activity.

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays (Figure 2) and yield and length of cDNA product.

Unit Definition: One unit of SuperScript® III RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as a template•primer (3).

Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C.

18080-044反转录酶III现货  产品规格:

Optimal Reaction Temperature:50° C
Final Product Size(s):12.3 kb or less
Reverse Transcriptase:SuperScript® III
Ribonuclease H Activity:Reduced
Form:Frozen
Format:Tube(s)
Quantity:10,000 units
Template:ssRNA
Sensitivity:Medium
Product Size:10,000 units
Concentration:200 U⁄µl
Enzyme Function:RNA dependent DNA Polymerase
Shipping Condition:Dry Ice
Number of Reactions:50 Reactions

 

 

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